TABLE 3.
P1 transductional mapping of λplacMu55(Kan) insertionsa
λplacMu55(Kan) allele(s) (min) | Donor marker (min) | % Linkage |
---|---|---|
apaG11 (1.1) | zab-3051::Tn10 (1.8) | 22 |
argP202, −203 (65.9) | ΔdsbC::Cm (65.4) | 12-33 |
galP::Tn10 (66.5) | 16-19 | |
mutY::Tn10 (66.8) | 6-10 | |
glnE463, −464, −465 (68.9) | metC162::Tn10 (67.9) | 11-16 |
proU610, −611 (60.4) | zga-900::Tn10 (60.3)b | 67-100 |
In each cross, linkage was determined as the proportion of Kans derivatives among the transductants inheriting the donor marker (chloramphenicol resistance in the case of ΔdsbC::Cm and tetracycline resistance in the case of all other markers). For the loci with two or more λplacMu55(Kan) insertion alleles, the range of linkage values obtained is indicated.
The zga-900::Tn10 insertion was designated zfi-900::Tn10 in a previous study (17).