FIG. 2.

In vivo activity of the RrmJ mutants. Polysome profiles of rrmJ deletion strain HB23 expressing no RrmJ or expressing wild-type RrmJ or the mutant proteins from a plasmid to wild-type protein levels. The ribosome profiles were analyzed under either associating, nonstringent salt conditions (10 mM MgCl₂, 100 mM NH₄Cl) (left panel) or dissociating, stringent salt conditions (1 mM MgCl₂, 200 mM NH₄Cl) (right panel). The ribosome profiles of lysates prepared from the individual strains (dark broken lines) are compared to the polysome profile of an rrmJ deletion strain expressing wild-type (wt) RrmJ (light broken lines).