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Western blot analysis of HA/protease expression in V. cholerae mutants. Supernatants from V. cholerae strains grown to saturation in TSB medium were concentrated in Centricon-10 centrifugal filters (Amicon Bioseparations), subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12% polyacrylamide) and transferred to a polyvinylidene difluoride membrane. Lanes: 1, C7258; 2, AJB19; 3, N16961; 4, AJB216; 5, AC-V66; 6, AJB231; 7, DSM-V491; 8, pure HA/protease.
