FIG. 2.

Glycolytic pH profile of S. sobrinus 6715. Cells were collected from a steady-state chemostat held at pH 7.0 (squares) or 5.0 (circles), washed once with ice-cold water, and resuspended in a 50 mM KCl/1 mM MgCl₂ solution. Glucose was added to the cell suspension to initiate glycolysis, and pH drops were continuously monitored for 50 min. Data points were collected every 10 s, and data for every 2 min 30 s are presented. The graph shown is a representative of five independent experiments.