FIG. 6.

Induction of S. sobrinus 6715 dnaK mRNA and DnaK in response to environmental acidification. (A) Slot blot of total RNA isolated from cells grown in the chemostat under the conditions indicated (s.s., steady-state). Hybridization was performed with an internal fragment of the S. sobrinus dnaK gene. RNase-treated samples were used as controls (data not shown). (B) Western blot analysis of DnaK with a polyclonal antibody against S. pyogenes DnaK (1:1,000). (C) Bar graph of DnaK levels as measured by densitometry.