FIG. 2.

Stabilization of RNA II by RNA I. Samples were taken from cultures at time intervals following the addition of rifampin to stop transcription initiation. RNA was purified, fractionated, transferred to nylon membranes, and probed with radiolabeled oligonucleotides specific for RNA I, RNA II, and E. faecalis 5S rRNA, as described in Materials and Methods. The 5S rRNA probe was added as a loading control. Time points are 0, 5, 10, 20, and 40 min after the addition of rifampin, shown from left to right on all gels. (A) Stabilization of RNA II in trans. Lanes 1 to 5, OG1X(pDAK611, pAM401); lanes 6 to 10, OG1X(pDAK611, pDAK704). The lower panel shows an overexposure of the RNA II bands. (B) Stabilization of RNA II in cis. Lanes 1 to 5, OG1X(pDAK102); lanes 6 to 10, OG1X(pDAK102Δ).