TABLE 1.

Plasmids

Plasmid	Relevant phenotype	Marker	Reference or source
Vectors
pAM401	E. coli-E. faecalis shuttle vector with pACYC184 and pIP501 replicons	Cmr, Tcr	53
pDL278	E. coli-E. faecalis shuttle vector with pVA380-1 replicon	Spr	32
pGEM-T	E. coli TA cloning vector	Ampr	Promega
pGEM-T Easy	E. coli TA cloning vector	Ampr	Promega
pSPORT1/2	E. coli cloning vector	Ampr	Gibco/BRL
par clones
pDAK606	pDL278 carrying the wild-type par locus	Spr	This work
pDAK607	pAM401 carrying the wild-type par locus	Cmr	50
pDAK608	pSPORT1 containing the RNA II gene	Ampr	50
pDAK611	pDL278 carrying the RNA II gene	Spr	50
pDAK616	pSPORT2 containing the RNA II gene antisense to the T7 promoter	Ampr	This work
pDAK704	pAM401 carrying the RNA I gene	Cmr	21
pDAK734	pAM401 carrying the 19-stop mutation of the RNA I gene	Cmr	This work
pDAK752	pAM401 carrying the IC mutant of the RNA I gene	Cmr	This work
pDAK901	pGEM-T containing the RNA I gene for in vitro transcription	Ampr	21
pDAK902	pGEM-T containing the RNA II gene for in vitro transcription	Ampr	21
pAD1 minireplicons
pDAK102	pAD1 minireplicon in pAM201 replicon probe vector with pACYC184 replicon	Cmr	47
pDAK102Δ	pDAK102 deleted for the RNA I gene	Cmr	This work
pDAK2300K	pAD1 minireplicon marked with Tn917	Emr	48