TABLE 3.
Stability of RNA II in the presence and absence of RNA I under various vector contexts
| RNA, and context (plasmid[s])a | Half-life (min)b
|
|
|---|---|---|
| RNA I | RNA II | |
| RNA II, high (pDAK611, pAM401) | — | 8.1 ± 0.8 |
| RNA I and II, high, in trans (pDAK611, pDAK704) | >40 | >40 |
| RNA I and II, low, in cis (pDAK2300K) | 60 ± 7 | 15.7 ± 2.6 |
| RNA I and II, low, in cis (pDAK102) | >40 | 16.2 ± 1.5 |
| RNA II, low, in cis (pDAK102Δ) | — | 4.1 ± 0.4 |
| RNA I and II, high, in cis (pDAK607) | >40 | >40 |
a
High, high copy number; low, low copy number.
b
Half-lives shown are an average of three independent experiments. —, not applicable.