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Discrepancy in mass of VirB10 expressed in C. jejuni and E. coli. Immunoblot of recombinant VirB10 isolated from E. coli and glycine extracts from C. jejuni DB179 and isogenic virB10 mutant. Protein samples were separated on a 10% acrylamide gel. Blots were incubated with an anti-VirB10 antiserum at 1:50,000 dilution. Lane 1, DB179; lane 2, DB179 (pVir/virB10::Km); lane 3, recombinant VirB10.
