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. 2004 Oct;186(19):6656–6660. doi: 10.1128/JB.186.19.6656-6660.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 1. — Binding of NorR to the norR-norV intergenic region. (A) Gel mobility shift assays contained a ³²P-labeled 362-bp EcoRI-BamHI fragment spanning the norR-norV intergenic region. NorR-retarded species are labeled A and B. Concentrations (nanomolar) of NorR were 0 (lane 2), 1 (lane 3), 20 (lane 4), 40 (lane 5), 60 (lane 6), 80 (lane 7), 100 (lane 8), 120 (lane 9), 140 (lane 10), 160 (lane 11), 180 (lane 12), 200 (lane 13), and 500 (lane 14). Lane 1 contained 1 μM IHF; the IHF-bound species is labeled C. A 360-bp fragment containing the nifH promoter (lane 15) was not bound by 500 nM NorR (lane 16). (B) Three independent gel mobility shift experiments (including the example shown in panel A) were quantified with a Fujix BAS 1000 phosphorimager. The total amount of retarded DNA is plotted as a percentage of the radioactivity present in each lane.