TABLE 4.
Impact of 2-aminobutyrate on salicylate, Dha, and pyochelin formation
| Strain | Genotype | Growth mediuma | Salicylate concn (nmol/ml)b | Dha concn (nmol/ml)b | Pyochelin concn (nmol/ml)b |
|---|---|---|---|---|---|
| PAO1 | Wild type | GGP | <8.0 | 87 ± 16 | 869 ± 111 |
| PAO1 | Wild type | GGP + 2-ABA | <8.0 | 62 ± 10 | 552 ± 28 |
| PAO6339 | ΔpchC | GGP | 708 ± 100 | 70 ± 4 | 422 ± 57 |
| PAO6339 | ΔpchC | GGP + 2-ABA | 87 ± 40 | <1.2 | 40 ± 22 |
a
GGP medium (30 ml) containing, where indicated, dl-2-aminobutyrate (2-ABA) at a final concentration of 5 mM, was inoculated with 0.1-ml portions of cultures grown in GGP medium. After incubation at 37°C and 220 rpm for 38 h, supernatants were extracted and analyzed for salicylate, Dha, and pyochelin by HPLC. The values are the means ± standard deviations for three parallel experiments.
b
Concentration in culture supernatant.