Figure 1.

Requirement of amphiphysin 1 for vesicle formation by brain cytosol. (A–E) Vesicle formation quantified by the DLS assay. Data represented are the relative distribution in numbers of formed vesicles. Large unilamellar liposomes were incubated in the presence of 200 μM GTP and 2 mM ATP under the conditions indicated. (F) Percentage of small vesicles formed in each incubation condition. Vesicles smaller than 200 nm in diameter were defined as small vesicles. Values represent the mean±s.d. for more than three independent measurements. Amph=amphiphysin 1; Amph KO cyto.=amphiphysin knockout mice brain cytosol; WT cyto.=wild-type mice brain cytosol.