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. 2004 Aug 12;23(17):3538–3547. doi: 10.1038/sj.emboj.7600356

Figure 3.

Figure 3

MBF1 binds a conserved basic motif. (A) GST pull-down assay showed a direct interaction of a GST-MBF1 fusion protein with the His-tagged D-Jun bZIP domain (J) but not with the same region of D-Fos (F). (B) Alignment of basic regions in the yeast bZIP factor GCN4, a nuclear receptor Ftz-F1, and the human and Drosophila AP-1 family members. All proteins except D-Fos have been shown to bind MBF1. The black boxes indicate arginine residues in GCN4 and Ftz-F1, known to be required for MBF1 binding; the corresponding basic residues in Fos and Jun are shaded. The arrow points to the critical redox-sensitive cysteine in AP-1 proteins.