Figure 8.

MBF1-deficient mutants are sensitive to oxidative stress. (A) Equal numbers of first instar larvae of the mbf1 mutant and rescued lines were placed on diet containing indicated concentrations of H₂O₂ and numbers of emerging adults were counted. Values above bars indicate the numbers of larvae. (B) Adult males (30 per vial) were exposed to H₂O₂ either directly (solid lines) or after reducing their catalase activity by feeding 5 mM aminotriazole for 60 h (broken lines), and their lifetime was recorded. The open symbols denote flies possessing at least one copy of mbf1⁺. Line P(mbf1⁺)/P(mbf1⁺); +/+ contains four copies of mbf1⁺. The numbers of flies tested per genotype ranged between 120 and 540, and the resistance of each genotype was tested at least three times.