Figure 6.

(A) E47 expression of DMS53 cell clones, stably overexpressing E47, was analyzed by immunoblotting and is shown in the upper panel. Parental and two independent clones (E10 and E17) were treated for 2 or 4 h with 5 nM BMP-2 and expressions of endogenous Mash1 and Id1 were analyzed by immunoblotting (lower panel). (B) Wild-type or Id1−/− MEFs were transfected with the indicated expression constructs. After 24 h, cells were splitted and treated with 10 nM BMP-2 for 3 h. Expressions of Mash1, Id1 and tubulin were analyzed by immunoblotting.