Figure 8.

(A) HEK-293 cells, transfected with the wild-type or mutant Mash1 constructs, with or without E47 expression construct as indicated, were labeled with [³⁵S]methionine and Mash1 was immunoprecipitated as above. (B) C17.2 cells were cotransfected with a TH promoter-driven reporter construct and the indicated combinations of wild-type and mutant Mash1 with (filled bars) or without (empty bars) E47 expression vectors. The results are expressed as mean±s.e.m. of triplicates from five independent transfections. (C, D) Pulse-chase assays and quantification were performed in HEK-293 cells, transfected with the constructs indicated, as described in Figure 4B.