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. 2004 Aug 19;23(17):3505–3515. doi: 10.1038/sj.emboj.7600361

Figure 1.

Figure 1

Characterization of BM CD34-derived DC from WT and PI3Kγ−/− mice. Immature DC (iDC) and mature (20 ng/ml TNF-α for 24 h) DC (mDC) were generated from CD34+ BM precursor cells in vitro. (A) Western blot analysis; (B, C) FACS analysis of the membrane phenotype; (D, E) MLR using DC from WT and PI3Kγ−/− mice; (F, G) cytokine determination in culture supernatants (24 h): CD40L (DC:J558, 4:1), LPS (100 mg/ml), SAC (1/100) Candida albicans (100 μg/ml); (H) FITC–dextran uptake; (I, J) RPA analysis of chemokine and chemokine receptor expression. Each experiment is representative of 3–5 independent DC cultures.

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