Figure 4.

Impaired migration of cutaneous DC in PI3Kγ−/− mice. (A) Number of MHC II⁺ DC/skin explant, emigrated into the culture medium after 48 h (medium, 20 ng/ml TNF-α, 100 ng/ml CCL21). (B) Number of WT MHC II⁺ DC (for each explant) emigrated in the presence of Wortmannin (30 nM) or pertussis toxin (600 ng/ml). (C) Number of FITC⁺/CD11c⁺ DC in lymph nodes following FITC skin painting. The results are the mean±s.e.m. of one representative experiment (eight mice per group) out of the three performed. (D) Total lymph node cellularity was evaluated in control mice and after FITC skin painting. The results are the mean±s.e.m. of one representative experiment (eight mice per group) out of the four performed (^**P<0.01, ^***P<0.001).