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Selective reduction of lymph node CD8α− DC in PI3Kγ−/− mice. DC subsets were characterized in peripheral lymph nodes from WT and PI3Kγ−/− mice. MHC-class II+ DC were gated and stained for CD11c and CD8α. Plasmacytoid DC (P-DC) were identified as B220+/Gr-1+ cells within a CD11c+/CD11b− population. The results are expressed as the percentage of MHC-class II+-positive cells (**P<0.01; n=7). The results for P-DC are the average of two experiments.
