Figure 4. ZIKV Infection Induces an Antiviral Response in HCs.
HCs from five donors were infected with ZIKV (PR 2015) at an MOI of 1, and antiviral gene expression determined by qRT-PCR. Gene expression data are represented as fold change relative to time-matched, mock-infected controls (gene expression normalized to GAPDH − ΔΔCT method). Individual donors are depicted as separate bars, organized from donor 1 to donor 5, within each time point block. Viral titers determined in Figure 1 are represented as a separate heat map below each group of genes. (A) shows type I IFNs, (B) shows RIG-I-like receptors, and (C) shows antiviral effector genes. hpi, hours post-infection.