Figure 1. IL-17 and IFN-γ responses in CD4⁺ T cell cultures with different stimuli.

Purified CD4⁺ T cells were cultured with allogeneic monocytes or MDDCs at a T cell/APC ratio of 5:1 or stimulated with a combination of anti-CD3 (2 µg/ml; eBioscience) and anti-CD28 (2 µg/ml; eBioscience) Abs. At d 5 and 13, these CD4⁺ T cells were stimulated with PMA and ionomycin, followed by intracellular staining with anti-IL-17 and IFN-γ Abs. The frequencies of IL-17⁺ cells and IFN-γ⁺ cells were determined by flow cytometry. (A) Dot plots from one representative subject showing IL-17 and IFN-γ expression in the CD4⁺ T cells. (B–D) Cumulative data showing the percentages of total IL-17⁺ (B, left panel) and IFN-γ⁺ (B, right panel), single-positive IL-17⁺ (C, left panel), single-positive IFN-γ⁺ (C, right panel), and double-positive IL-17⁺IFN-γ⁺ (D) cells out of CD4⁺ T cells in the cultures from different donors. The red bars represent means. P values were calculated using the unpaired t test. *P < 0.05, **P < 0.01.