Figure 4. IL-17 and IFN-γ responses induced in memory, naïve, and total CD4⁺ T cells cocultured with HIV-treated monocytes.

Memory CD45RA⁻ CCR6⁺ CD4⁺ T cells, naïve CD45RA⁺ CD4⁺ T cells, and total CD4⁺ T cells were cocultured with allogenic HIV-treated monocytes at a T/APC ratio of 5 to 1 for 13 d. At d 13, the cells were stained with anti-IL-17 and IFN-γ Abs after PMA and ionomycin treatment and analyzed by flow cytometry. (A) Dot plots from one representative subject showing IL-17 and IFN-γ expression in the memory (top panels), naïve (middle panels), and total CD4⁺ T cells (lower panels). (B) Fold-changes of the IL-17⁺ CD4⁺ T cell frequencies in the cocultures with HIV-1-treated monocytes over those of mock-treated controls. Means ± se from 2–3 different donors are shown.