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. 2016 Aug 4;101(1):239–251. doi: 10.1189/jlb.2A1215-572R

Figure 6. Rap2 KD in HL-60 cells exhibit chemotaxis defects.

Figure 6.

(A) Rap2 was knocked down by using Rap2shRNA targeting virus particles (n = 3). Neither ArrB1 nor Rap1 expression was affected by Rap2shRNA. Quantification of Rap2 KD was normalized to actin. (B) Montage showing the path of chemotaxing NTshRNA and Rap2shRNA-infected cells was monitored by EZ-TAXIScan for 0–5 min (upper) or 5–30 min (lower) to show that Rap2shRNA-infected cells exhibited an early migratory defect. (C) Chemotaxis parameters, net path length, migration speed, directionality, and roundness were determined during the first 5 and 5–30 min and quantified using DIAS software.