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. 2016 Aug 4;101(1):239–251. doi: 10.1189/jlb.2A1215-572R

Figure 8. A schematic diagram illustrating the regulation of Rap2GTPase by ArrB1 in neutrophil-like HL-60 cells.

Figure 8.

FPR activation leads to the dissociation of the active Rap2 GTPase-Ral-GDS complex from ArrB1. fMLP binds to the FPR at the plasma membrane. (1) Heterotrimeric G protein dissociates from the FPR after agonist binding. (2) GRK phosphorylates the receptor and creates a high-affinity binding site for ArrB1. (3) The translocation of the ArrB1-Ral-GDS-Rap2 GDP complex to the membrane. (4) ArrB1 binding to the receptor leads to dissociation of the Ral-GDS and Rap2-GDP complex, and Ral-GDS acts as a GEF for Rap2, which leads to activation of Rap2. (5) Required for cell adhesion and migration in response to fMLP stimulation.