Figure 5. DLE activates inflammatory genetic pathways in DCs.
RNA-seq heat map for 1e6 DCOVA incubated for 18 h with 1e6 ceq of indicated DLE compared with diluent control. Selected for display were genes that had statistically significant differential expression [difference of estimation (Diff. of Est.); log2 ratio > 2 in the hypothesized direction, and FDR < 0.05] for all 3 tests: OVA:DLE versus HKCA:DLE, OVA:DLE versus diluent, and CD8-enriched (CD8enr) OVA:DLE versus OVA:DLE. The table of log2 ratio values was sorted, according to the value for CD8enr OVA:DLE versus OVA:DLE, and colorized as indicated in the legend. Data shown are combined from 3 independent experiments; significance determined by ANOVA.