Figure 3. SiglecF⁺Gr1^hi eosinophils are detected in response to Af-sensitization and challenge in wild-type (WT) BALB/c, but not eosinophil-deficient, ΔdblGATA mice.

SiglecF⁺Gr1⁻ eosinophils (A) and SiglecF⁺Gr1^hi eosinophils (B) in single-cell lung suspensions from Af-sensitized and -challenged mice, as described in Fig. 1; **P < 0.01 Af-sensitization and Af-challenge vs. Af-sensitization and PBS challenge, ^††P < 0.01 PVM infected vs. diluent control, 2-way ANOVA, n = 3–5 mice/group. C. SiglecF⁺Gr1^hi eosinophils are an independent population and represent ∼5% of the CD45⁺CD11c⁻Gr1⁺ cells isolated from lungs of Af-sensitized and -challenged BALB/c mice. (D) SiglecF⁺Gr1^hi cells cannot be detected in Af-sensitized and -challenged, eosinophil-deficient ΔdblGATA mice.