Table 1.

Cytokines and chemokines detected in lysates of SiglecF⁺Gr1⁻ vs. SiglecF⁺Gr1^hi eosinophils from lungs of allergen-challenged mice

Cytokines	Abbreviations and alternative names		In SiglecF+Gr1hi eosinophilsa		In SiglecF+Gr1− eosinophilsb		Change
CC chemokine ligand 1	CCL1	I-309	−		+		↑
CC chemokine ligand 12	CCL12		+		+		−
CC chemokine ligand 17	CCL17		−		++		↑
CC chemokine ligand 2c	CCL2	MCP-2	−	0	+	5.5	↑
CC chemokine ligand 3	CCL3	MIP-1α	−		+		↑
CC chemokine ligand 4	CCL4	MIP-1β	−		+		↑
CXC chemokine ligand 10c	CXCL10	IP-10	−	0	++	1680	↑
CXC chemokine ligand 12	CXCL12	SDF-1	+		++		↑
CXC chemokine ligand 13c	CXCL13	BCA-1	++	3.9	−	0	↓
Interferon-γ	IFN-γ		+		−		↓
Interleukin-10d	IL-10			322		1220	↑
Interleukin-13c	IL-13		+	71.7	−	0	↓
Interleukin-16	IL-16		−		+		↑
Interleukin-17d	IL-17		+	34.2	++	47.1	↑/−
Interleukin-1α	IL-1α	IL1-F1	+		+		−
Interleukin-1β	IL-1β	IL1-F2	+		+		−
Interleukin-2	IL-2		+		++		↑
Interleukin-23	IL-23		+		++		↑
Interleukin-27c	IL-27		+	4.8	−	0	↓
Interleukin-3	IL-3		+++		++		↓
Interleukin-7	IL-7		+		+		−
Colony stimulating factor-1	CSF-1	M-CSF	+		+		−
Transforming growth fact βd	TGF-β			0		0	−
Tumor necrosis factor α	TNF-α		−	29	+	19	↑/−

Eosinophils (CD45⁺CD11c⁻SiglecF⁺Gr1⁻ and CD45⁺CD11c⁻SiglecF⁺Gr1^hi) were isolated by FACS from single-cell suspensions from lung tissue of mice challenged with a filtrate from Aa (see Fig. 4A). Lysates were prepared as described in the “Methods” section, with 300 µg of each lysate used in simultaneous probes of Ab-embedded membranes for detection of total cytokine contents. Cytokines were detected on the profiler as the signal intensity of the sample/signal intensity of the reference. Cytokines included in the evaluation but not detected in either eosinophil lysate included G-CSF, CCL11, GM-CSF, IL-4, CXCL9, and CCL5. Values shown are in units of picograms/10⁶ cells. −, no difference from reference; +, ++, and +++, serial multipliers over the reference standard.

^aPurity determined by back sorts at 97.5%.

^bPurity determined by back sorts at 99.7%.

^cPrimary values for CCL2, CXCL10, CXCL13, IL-13, IL-17, and IL-27 determined by profiler were confirmed by ELISA.

^dValues for IL-10 and TGFβ were generated by ELISA.