Table 1.
Primary antibody | Type | Antigen retrieval method | Working dilution | Incubation parameters |
---|---|---|---|---|
CD3 (IS503, Dako) | Rabbit polyclonal | Heating under pressure in 10 mM Tris-EDTA buffer (pH 9), 15 min | 1:600 | 2 h at RT |
iNOS (AHP477, Serotec) | Rabbit polyclonal | None | 1:3000 | 2 h at RT |
MAC387 (MS-148-R7, Neomarkers) | Mouse monoclonal | Proteinase K, 1:500 / 15 min | RTU | 2 h at RT |
Active Caspase-3 (G7481, Promega) | Rabbit polyclonal | Heating under pressure in 1 mM EDTA buffer (pH 8), 2 min | 1:500 | 2 h at RT |
CD79α (IR621, Dako) | Mouse monoclonal | Heating under pressure in target retrieval solution high pH (Dako), 15 min | RTU | 2 h at RT |