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. 2016 Nov 24;12(12):1448–1460. doi: 10.7150/ijbs.14967

Table 1.

Primary antibodies and immunohistochemical procedures used in the histopathological studies.

Primary antibody Type Antigen retrieval method Working dilution Incubation parameters
CD3 (IS503, Dako) Rabbit polyclonal Heating under pressure in 10 mM Tris-EDTA buffer (pH 9), 15 min 1:600 2 h at RT
iNOS (AHP477, Serotec) Rabbit polyclonal None 1:3000 2 h at RT
MAC387 (MS-148-R7, Neomarkers) Mouse monoclonal Proteinase K, 1:500 / 15 min RTU 2 h at RT
Active Caspase-3 (G7481, Promega) Rabbit polyclonal Heating under pressure in 1 mM EDTA buffer (pH 8), 2 min 1:500 2 h at RT
CD79α (IR621, Dako) Mouse monoclonal Heating under pressure in target retrieval solution high pH (Dako), 15 min RTU 2 h at RT