Table 2.
Primers used to analyze cytokine gene expression by RT-qPCR.
| gene | Fwd PRIMER seq (length) | Rev PRIMER seq (length) | Length product (pb) | Anneal temperature (ºC) |
Primer concentration (μM) |
Product Tm (ºC) |
|---|---|---|---|---|---|---|
| Actin | GACGATGCTCCCCGGGCTGTATTC (24) | TCTCTTGCTCTGGGCCTCGTCACC (24) | 117 | 60 | 5 | 87 |
| IL-1-β | CAACCAACAAGTGATATTCTCCATG (25) | GATCCACACTCTCCAGCTGCA (21) | 152 | 60 | 5 | 83 |
| IL-6 | ACCACGGCCTTCCCTACTTCAC (22) | TCCTCATTTCCACGATTTCCCAG (23) | 134 | 60 | 5 | 81 |
| IL-12p40 | ACAGCACCAGCTTCTTCATCA (21) | TCTTCAAAGGCTTCATCTGCA (21) | 75 | 60 | 5 | 82 |
| IFNγ | GCGTCATTGAATCACACC (18) | GGACCTGTGGGTTGTTGACC (20) | 105 | 60 | 5 | 81 |
| TNFα | AGCCCACGTCGTAGCAAACCAC (22) | ATCGGCTGGCACCACTAGTTGGT (23) | 121 | 60 | 5 | 88 |