Table 1.
Primer sequences used for the OVX-BMSCs.
| Gene Target | Primers sequence (5'-3') | Product size (bp) |
|---|---|---|
| Col-I | Forward: GGTCCCAAAGGTGCTGATGG | 182 |
| Reverse: GACCAGGCTCACCACGGTCT | ||
| ALP | Forward: GTCCCACAAGAGCCCACAAT | 172 |
| Reverse: CAACGGCAGAGCCAGGAAT | ||
| Runx2 | Forward: ATCCAGCCACCTTCACTTACACC | 199 |
| Reverse: GGGACCATTGGGAACTGATAGG | ||
| OCN | Forward: CAGTAAGGTGGTGAATAGACTCCG | 172 |
| Reverse: GGTGCCATAGATGCGCTTG | ||
| BMP-2 | Forward: GAAGCCAGGTGTCTCCAAGAG | 122 |
| Reverse: GTGGATGTCCTTTACCGTCGT | ||
| OPG | Forward: GTCCCTTGCCCTGACTACTCT | 250 |
| Reverse: GACATCTTTTGCAAACCGTGT | ||
| RANKL | Forward: CCCATCGGGTTCCCATAAAGTC | 146 |
| Reverse: GCCTGAAGCAAATGTTGGCGTA | ||
| BSP | Forward: AGAAAGAGCAGCACGGTTGAGT | 175 |
| Reverse: GACCCTCGTAGCCTTCATAGCC | ||
| VEGF | Forward: GGCTCTGAAACCATGAACTTTCT | 165 |
| Reverse: GCAGTAGCTGCGCTGGTAGAC | ||
| ANG-1 | Forward: GGACAGCAGGCAAACAGAGCAGC | 130 |
| Reverse: CCACAGGCATCAAACCACCAACC | ||
| GAPDH | Forward: GGCAAGTTCAACGGCACAGT | 76 |
| Reverse: GCCAGTAGACTCCACGACAT |