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Primer sequences used for the HUVECs.
| Gene Target | Primers sequence (5'-3') | Product size (bp) |
|---|---|---|
| ET-1 | Forward: CAGAGGCGATCACAGCAACCA | 296 |
| Reverse: CAAGGAGCAGGAGCAACG | ||
| VEGF | Forward: GAGTACCCTGATGAGATCGAGT | 193 |
| Reverse: ATTTGTTGTGCTGTAGGAAGCT | ||
| PAI-1 | Forward: TGCCCTCTACTTCAACGG | 307 |
| Reverse: GTCGGTCATTCCCAGGTT | ||
| t-PA | Forward: CCAGATCGAGACTCAAAGCC | 119 |
| Reverse: GACCCATTCCCAAAGTAGCA | ||
| GAPDH | Forward: CGTATTGGGCGCCTGGTCAC | 248 |
| Reverse: ACGTACTCAGCGCCAGCATCG |
