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. 2016 Nov 26;12(12):1533–1543. doi: 10.7150/ijbs.15943

Figure 1.

Figure 1

COX-2 in TAMs promotes migration and invasion of breast cancer cells. (A) The expression of COX-2 in TAMs transfected with adenoviral COX-2 or siRNA COX-2 was checked by western blot. β-actin was used as an internal loading control. The blots shown are representative of six independent experiments. (B) For the wound healing assay, confluent monolayers of MCF-7 and MDA-MB-468 cells co-cultured with or without (Alone) TAMs transfected with adenoviral COX-2 or siRNA COX-2 for 7 days were scarred. The migration of the cells into the wound zone was monitored by microscopy over a 12-h period. The upper panel shows the wound healing assay in MCF-7 cells. White lines indicate the scraped zone. (C) and (D) The transwell invasion assay. After MCF-7 and MDA-MB-468 cells were co-cultured with or without (Alone) TAMs (C) or MDMs (D) transfected with adenoviral COX-2 or siRNA COX-2 for 7 days, the cells that migrated to the lower chamber or invaded through the Matrigel were fixed, stained, and counted using a light microscope. Four random fields per filter were scanned for the presence of cells on the lower side of the membrane. The upper panel shows the cell invasion assay in MCF-7 cells (original magnification, ×100). All experiments were performed thrice in triplicate. The data are presented as the mean ± SD. *p < 0.05 and **p < 0.01 (versus Alone group).