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. 2016 Nov 26;12(12):1533–1543. doi: 10.7150/ijbs.15943

Figure 3.

Figure 3

COX-2 in TAMs enhances TAMs-induced EMT in breast cancer cells. (A) EMT markers in MCF-7 and MDA-MB-468 cells were assessed by Western blot. β-actin was used as an internal loading control. The blots shown are representative of six independent experiments. (B) Immunostaining showed the downregulation of E-cadherin and upregulation of Vimentin in MCF-7 and MDA-MB-468 cells (original magnification: 400×). The nucleus is stained with DAPI (blue), E-cadherin is stained with DyLight 549 (red), and Vimentin is stained with DyLight 488 (green). The experiments were performed thrice in triplicate. The data are presented as the mean ± SD. **p<0.01 (versus Alone group). (C) EMT markers in MCF-7 and MDA-MB-468 cells co-cultured with or without (Alone) TAMs for 7 days were assessed by Western blot. β-actin was used as an internal loading control. The blots shown are representative of six independent experiments. (D) Correlation of COX-2+ TAMs and Vimentin in breast cancer tissues (n = 160) was analyzed by Pearson's correlation analysis.