Figure 8.

Effects of MHY2256, salermide, and doxorubicin on the growth of MCF-7 tumors in nude mice. Mice with established tumors were randomized into four groups. Vehicle control, doxorubicin (4 mg/kg/week, i.p.), salermide (30 mg/kg/week, i.p.) or MHY2256 (5 mg/kg, twice/week, i.p.) were administered to the tumor-bearing mice. (A) The mean tumor volumes for each treatment group are indicated. (B) Each bar represents the inhibition rate (% of control) of mean tumor weight. The results are presented as the mean ± SEM per group. Statistical analysis was performed using one-way analysis of the variance (ANOVA) followed by Bonferroni's multiple comparison test. *p<0.05, **p <0.01 indicate significant differences between control and treatment groups. (C) The tumors were fixed in 10% formalin and embedded in paraffin. Immunohistochemical staining for Ki-67 were measured in tumors. Magnification x200. Scale bar = 50 μm. The representative images were recorded under a 40x objective lens. (D) Expression levels of p53, acetylated p53, MDM2, Bax, and Bcl-2 were measured in tumor tissues using Western blot analysis. Western blots are representative of three independent experiments.