Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 19;215(6):801–821. doi: 10.1083/jcb.201604112

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Lawrence et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License(Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — RAD-51 and RPA are altered after HU treatment in the absence of UNC84. (A) RAD-51–positive nuclei in wild type (WT), unc-84(n369), unc-84(n369);cku-70(tm1524), or cku-70(tm1524) in the absence of or after recovery from 5 mM HU. n > 30. (B) Number of RPA-1::YPF foci per nucleus in wild type, unc-84(n369), unc-84(n369);cku-80(RNAi), or cku-80(RNAi) in the absence of or after recovery from 5 mM HU. n > 30. (C) MAD-2 (red) staining in wild-type or unc-84(n369) germlines after 25 mM HU treatment counterstained with DAPI (blue). (D) High-resolution SIM images of a single nucleus from unc-84(n369) and wild-type worms treated with 25 mM HU and stained with RAD-51 (red), NPC (Mab414; green), and DAPI (blue). Next to each image is the mean number of RAD-51 foci observed in each genotype as well as the mean area of each RAD-51 focus (n = 4 germlines). Images represent a projection of three z sections. The scatterplot indicates the distance in nanometers between NPC and RAD-51 foci in unc-84(n369) and wild-type worms after HU. Bars, 5 µm. Error bars indicate 95% CI. *, P < 0.05; **, P < 0.01; ***, P < 0.0001, when compared with unc-84(n369) single mutants.