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. 2016 Dec 19;215(6):857–874. doi: 10.1083/jcb.201607039

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© 2016 Nguyen et al.

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Figure 2. — Analysis of autophagosome initiation and nucleation events in Atg8 knockout lines during PINK1/Parkin mitophagy. (A and D) Representative images of WT, LC3 TKO, GBRP TKO, and hexa KO cells expressing mCh-Parkin and GFP-ULK1 (A) or GFP-DFCP1 (D), immunostained for HSP60 and GFP after 3-h OA treatment (untreated images shown in Fig. S2, G and H). Quantification by image analysis of ULK1 foci structures per cell (B), DFCP1 structures per cell (E), and the proportion of mitochondrially associated (C) ULK1 foci and (F) DFCP1 structures in WT, LC3 TKO, GBRP TKO, and hexa KO cells. Data in B, C, E, and F are mean ± SD from three independent experiments using measurements from >80 cells per sample. *, P < 0.05; **, P < 0.005; ***, P < 0.001 (one-way ANOVA). Bars: 10 µm; (insets) 2 µm.