Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 19;215(6):857–874. doi: 10.1083/jcb.201607039

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Nguyen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Atg8s are crucial for correct acidification of sequestered cargo. (A and B) WT, LC3 TKO, GBRP TKO, and hexa KO expressing YFP-Parkin and mtKeima were either untreated or treated with OA for indicated times and analyzed for (A) lysosomal-positive mtKeima using fluorescence microscopy (550 nm mtKeima; see Fig. S5 A for 2- and 8-h images), and (B) FACS as the percentage of 561 nm mtKeima positive cells (see Fig. S5 B for FACS plots). (C and D) Hexa KO cells expressing mtKeima and doxycycline (Dox)–inducible HA-LC3C or HA-GBRPL1 were pretreated with OA for 8 h. After being incubated with Dox, Wort, and OA for 6 h, the cells were analyzed by confocal microscopy (C) and FACS (D) for lysosomal-positive mtKeima (see Fig. S5, C and D). Data in B and D are mean ± SD from three independent experiments. ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA). ns, not significant. Bars, 10 µm.