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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2015 Dec 29;102:120–129. doi: 10.1016/j.bcp.2015.11.024

Fig. 6. Effect of sgk2 on the interaction between Nedd4-2 and hOAT4.

Fig. 6

(a) COS-7 cells were transfected with hOAT4, Nedd4-2 and control vector or with hOAT4, Nedd4-2 and the constitutive active form of sgk2 (CA-sgk2). Transfected cells were then lysed, and hOAT4 was immunoprecipitated (IP) with anti-myc antibody, followed by immunoblotting (IB) with anti-Nedd4-2 antibody. (b) The same immunoblot from Fig. 6a was reprobed by anti-myc antibody. (c) Densitometry plot of results from Fig. 6a as well as from other experiments. The values are mean ± S.E. (n = 3). *P<0.05