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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2015 Dec 29;102:120–129. doi: 10.1016/j.bcp.2015.11.024

Fig. 8. Effects of Nedd4-2 ligase activity on sgk2 regulation of hOAT4 transport function.

Fig. 8

COS-7 cells were transfected with hOAT4, CA-sgk2 and control vector, or with hOAT4, CA-sgk2 and Nedd4-2, or with hOAT4, CA-sgk2 and ubiquitin ligase-dead Nedd4-2 mutant (Nedd4-2/C821A). Transfected cells were then measured for the uptake of [3H]-estrone sulfate (3-min uptake and 0.1 μM estrone sulfate). The data represent uptake into hOAT4-transfected cells minus uptake into mock cells (parental COS-7 cells). Values are mean ± S.E. (n = 3). *P<0.05, NS: statistically not significant.