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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2015 Dec 29;102:120–129. doi: 10.1016/j.bcp.2015.11.024

Fig 9. Effect of Nedd4-2-specifc siRNA-knock down of endogenous Nedd4-2 on sgk2 regulation of hOAT4 transport activity.

Fig 9

(a) COS-7 cells were co-transfected with hOAT4 and scrambled control siRNA or with hOAT4 and Nedd4-2-specific siRNA. The effectiveness of Nedd4-2-specific siRNA was tested by probing the lysis sample with anti-Nedd4-2 antibody. (b) COS-7 cells were co-transfected with hOAT4 and scrambled control siRNA or with hOAT4 and Nedd4-2-specific siRNA. The expression of the house-keeping protein β-actin was determined by probing the lysis sample with anti-actin antibody. (c) COS-7 cells were co-transfected with hOAT4 and scrambled control siRNA or with hOAT4 and Nedd4-2-specific siRNA. Transfected cells were then measured for the uptake of [3H]-estrone sulfate (3-min uptake and 0.1 μM estrone sulfate). The data represent uptake into hOAT4-transfected cells minus uptake into mock cells (parental COS-7 cells). Values are mean ± S.E. (n = 3). *P<0.05, NS: statistically not significant.