Figure 3.

Effects of the indicated treatments on Wnt/β-catenin pathway proteins in tumor-burdened mice. (a) Immunohistochemical staining for the indicated proteins in tumor tissue. (b) Mean optical densities of the indicated protein signals. MG: model vehicle control group; CTX: CTX-treated group; HA-H: high-dose helvolic acid; HA-L: low-dose helvolic acid; CG: combination of CTX + HA-L group. NG: indicates tissue from the MG mice, processed in the absence of primary antibody as a negative control. *P < 0.05 and **P < 0.01 vs. MG; ^#P < 0.05 vs. PG. Scale bar = 50 µm