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. 2016 Nov 14;8(12):1409–1420. doi: 10.15252/emmm.201506035

Figure 1. Daily systemic 4‐AP administration (10 μg/day, i.p.) improves functional recovery of crushed sciatic nerve.

Figure 1

  • A
    Daily 10 μg 4‐AP enhanced recovery of sciatic nerve motor function as compared with treatment with vehicle at 3 days post‐injury (dpi) through 8 dpi. *: day 3 (D3), P = 0.0131; D5, P = 0.0475; D8, P = 0.0472; ANOVA with post hoc comparisons using two‐tailed unpaired t‐test. n = 6.
  • B
    Daily 10 μg 4‐AP administration also enhanced recovery of nerve conduction velocity (NCV) as observed beginning at 21 days post‐injury, eventually restoring NCV to near‐normal values while NCV in vehicle‐treated mice remained less than half that of uninjured animals. *: D21, P = 0.0454; D28, P = 0.0487; D35, P = 0.0475; ANOVA with post hoc comparisons using two‐tailed unpaired t‐test. n = 5.
  • C, D
    Analysis of withdrawal latency in response to thermal (C) or mechanical (D) stimuli revealed that 4‐AP treatment did not worsen these diagnostics of neuropathic pain syndromes. In the case of response to thermal hyperalgesia, 4‐AP‐treated mice showed a significantly more rapid return to baseline levels. *: (C) D3, D5, D8: P < 0.001 saline versus baseline; D3, P = 0.006 4‐AP versus baseline; (D) D3, P = 0.014; D5, P = 0.008; saline versus baseline; D3, P = 0.006; D5, P = 0.0472; 4‐AP versus baseline; ANOVA with post hoc comparisons using two‐tailed unpaired t‐test. n = 10.
Data information: Data are presented as mean ± SEM and show a representative experiment from 2 to 3 repetitions.