Figure EV2. Selective expression of IP₃R3 in the motor nuclei of mouse brain.

• A–F
  Immunofluorescence staining of mouse brains from non‐transgenic mice. Sagittal sections of mouse brains were stained using anti‐Sig1R (white), βIII‐tubulin (red), and IP₃R3 (green) antibodies. Sig1R was expressed in most of the neurons tested. However, IP₃R3 was not expressed in cerebellum (A), hippocampus (B), or cortex (C), but specifically co‐localized with Sig1R in hypoglossal nucleus (D), facial motor nucleus (E), or motor nucleus of trigeminal (F), indicating selective expression in IP₃R3 in the motor‐related nuclei. Scale bars: 50 ?m. The white boxed areas were magnified in the images at bottom.