Figure EV4. Niacin acts through Tace to modulate myelination in Mtmr2 ^−/− cultures.

• A
  Quantitative RT–PCR analysis using mRNA from isolated rat Schwann cells transduced with Tace shRNA LV (three different harpins tested). NI, not infected; SCR, scramble shRNA LV. Representative of two independent experiments.
• B
  Quantification of Mbp‐positive myelin segments in wild‐type cultures transduced with Tace shRNA LVs as compared to NI and transduced with scramble shRNA. DRGs/coverslips: NI n = 6; SCR n = 4; sh#1 n = 4. P = 0.0181, nonparametric one‐way ANOVA, followed by Dunn's post hoc test.
• C
  Representative of Mtmr2 ^−/− explants transduced with scramble shRNA (GFP tag) shows that neurons were preferentially transduced by LVs when infection was made 1 day after plating the DRGs.
• D, E
  Representative confocal images of Mtmr2 ^−/− co‐cultures transduced using LV expressing shRNA scramble (SCR) or Tace shRNA #1 and treated or not treated (NT) using 5 mM niacin, with quantification in (E). N = 6 DRGs/coverslips per condition; 200 fibers scored per condition; P = 0.0425, nonparametric one‐way ANOVA, followed by Dunn's post hoc test.

Data information: Results in (A, B, and E) are mean ± SEM. Dunn's post hoc test, *P < 0.05.