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. 2016 Nov 23;113(50):E8141–E8150. doi: 10.1073/pnas.1617460113

Fig. 3.

Fig. 3.

B. adolescentis (BA)-driven intestinal Th17 responses are cognate. (A and B) BA-specific Th17 cells. Frequencies of SI-LP CD4+ T cells producing (A) IL-17A or (B) IFN-γ on overnight stimulation by splenic dendritic cells incubated with media alone or lysates prepared from the indicated bacterial species. +Anti–MHC-II (the blocking antibody M5/114.15.2) was added. (C) Frequency of SI-LP CD4+ T cells producing IL-17A on stimulation with media alone or BA lysate when T cells were isolated from mice colonized as indicated. (D) The degree of TCRVβ14 enrichment in SI-LP CD4+ T cells from SPF mice colonized as indicated, calculated as (percent of Vβ14+ cells in Th17 fraction)/(percent of TCRVβ14+ cells in non-Th17 fraction). Each symbol represents one mouse. Data are pooled from two to four independent experiments. Mean ± SEM. BF, B. fragilis; P + I, PMA and ionomycin. *P < 0.05 (Kruskal–Wallis test and Dunn’s multiple comparisons test); **P < 0.01 (Kruskal–Wallis test and Dunn’s multiple comparisons test); ***P < 0.001 (Kruskal–Wallis test and Dunn’s multiple comparisons test).