Fig. S4.

WNK1 depletion relocalizes UVRAG. WNK1 was knocked down by 20 nM siRNA in U2OS cells. After 3 d, the cells were treated with fresh medium for 4 h. Then the cells were analyzed by immunofluorescence. The UVRAG cellular distribution was calculated using CellProfiler software (74). For each cell, the median Euclidean distance between UVRAG-staining speckles and the center of nucleus was obtained and normalized to median cell radius. The resulting value was taken as the distance of UVRAG from the nucleus. (Scale bar, 150 pixels.)