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Hyperosmotic stress-induced autophagy is independent of WNK1. WNK1 was knocked down by 20 nM siRNA in HeLa cells. After 3 d, the cells were treated with medium containing 0.05–0.2 M NaCl (Also with 0.1% DMSO) for 4 h. Then the cells were analyzed by immunoblotting. n = 2.
