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. 2016 Nov 29;113(50):14348–14353. doi: 10.1073/pnas.1613999113

Fig. 5.

Fig. 5.

GEF-mediated GTPase switching monitored by K-Ras sensor. (A) Addition of SOScat to 200 nM EGFP–K-RasD30C–TF3 induces rapid nucleotide exchange in the presence of excess GTP (100 μM). (A, Inset) Fluorescence spectra of EGFP–K-RasD30C–TF3 before (black line) and after (orange line) nucleotide exchange. (B) Rate constants of nucleotide exchange depend on SOScat concentration. (B, Inset) Plot of observed rate constant against SOScat concentration, yielding the second-order rate constant for nucleotide exchange (kcat/Km). FRET was monitored by excitation set at 480 nm and emission at 580 nm.