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. 2016 Nov 30;113(50):14324–14329. doi: 10.1073/pnas.1608917113

Fig. 4.

Fig. 4.

Proposed reaction mechanism for NOV1. (A) Formation of ternary complex of Fe(III)-superoxo Lig13, O2 and alkene substrate, and contributions of deprotonation of 4′-OH by Y101 and K135 in activation of the substrate. (B) Intermediate formed prior to formation of a C–O bond. (C) Intermediate formed after formation of the first C–O bond. (D) Cleavage of the O–O bond and formation of the second C–O bond. (E) Cleavage of the C–C bond and restoration of the Fe(II) enzyme. (F) Reaction products.